| Preparation of DNA for Injection | Buffer and concentrations required |
| ES Cell Culture | References, reagents and conditions for ES cell culture, electroporation and expansion |
| PCR | Buffer reaction mix and sample prep for cells and tissue |
| PCR screening of mutant strains | Reagents and procedure for identification of targeted clones |
| Southern blot | Processing gel, transfer, probing and washing, and exposure to film |
| Southern blot screening of ES cell clones | Reference to protocol adapted to 96 well format |
| Transgenic Animal Procedures | Mouse procedures practiced by the transgenic facility |
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