The Genomics Resource provides access to self-service qPCR (real-time PCR) instruments located in DE-341. Three ABI 7900HT Real Time PCR Systems (two 96-well block and one 384-well block) are available 24 hours a day, with staff available to answer questions Mon-Fri (9AM-5PM).
Those new to qPCR technology, ABI instrumentation, or the Genetic Analysis Lab should schedule a one-on-one introductory instrument training session. Please contact the resource staff to schedule an appointment.
All information provided below is pertinent to Fred Hutchinson/UW Cancer Consortium members. For non-consortium members interested in services please contact the Genomics Resource at (206) 667-4470 or one of the individuals listed below.
ContactsTo reserve an instrument, those with a HutchNet ID and a fred account can use the online Instrument Scheduler. Those who do not have a HutchNet ID and fred account should contact the Genomics resource for assistance.
Evening hours must be scheduled by no later than 4PM the day of the desired reservation; weekend hours must be reserved by no later than 4 PM on the proceeding Friday. If you must cancel a reservation, please give as much advanced notice as possible (no less than 24 hr). If you start your run late and it extends beyond the end of your reservation, please be aware that there is a likelihood that your run will be aborted to accommodate the next reservation in the queue.
All reagents and consumable are the responsibility of the investigator. Common suppliers include ABI, Stratagene, Qiagen, and Invitrogen. All consumables (plates, caps, film) must be of optical quality. A centrifuge with a microtiter plate rotor is available and is located in the vicinity of the instruments.
All data files will be saved on the hard drive of the computer operating the particular instrument. Please create a folder on the hard drive and designate that folder in the Save As query that appears when you begin your run. Please transfer the file to the server once your experimental run has been completed.
The ABI machines generate a .sds file which can be analyzed only by SDS 2.2.2 software (PC-compatible only). Contact the resource to get a copy of the software or go to Genomics IT site to download a copy. (License agreements dictate that some of the software is available only from FHCRC based computers. If you are not at FHCRC and need access to the software, please contact Genomics and we will arrange access for you.)
